Monday, December 16, 2013

How to identify Riboflavin-5-phosphate sodium


Identification test: Riboflavin - 5 - phosphate sodium solubility soluble in water. Insoluble in ethanol. According to the OT - 42 method determination.
Than the sample rotation under 100 ℃ in the phosphorus pentoxide in vacuum drying after 5 h, with a 20% (W/V) hydrochloric acid as solvent, the preparation of 1.5% (W/V) sample liquid, then according to the conventional method. The [alpha] D20 shall be the + 38 ° ~ 42 °. Sodium salt in IT - 28 method determination of the sodium sulphate ash content.
Sample solution at 375 nm and 267 nm wavelength of maximum absorbance ratio is 0.30 ~ 0.34, at 444 nm and 26.7 nm ratio is 0.35 ~ 0.40.
Content analysis: content analysis shall be carried out under the direct light. Take a sample of 100 mg in a 500 ml volumetric flask brown, add water, 100 ml and 2.5 ml glacial acetic acid, water constant volume. Take the 20.0 ml liquid into a 200 ml volumetric flask, brown and 1.4% (W/V) sodium acetate solution 3.5 ml, constant volume with water. The determination of maximum absorbance of the solution at 444 nm (A).
The total amount of pigment (A x = 5000/328 (W) x 1.367
Type of W - sample quantity, g.
Purpose: vitamin drugs, used in the treatment of a variety of VB2 deficiency, but also can be used as a food additive

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