Introduction of Myoglobin ELISA

Principle of the test: The Myoglobin ELISA analysis is based on the assumption of a solid appearance enzyme-linked immunosorbent assay. The appraisal arrangement utilizes a different monoclonal antibiotic directed adjoin a audible antigenic account on the myoglobin molecule. Abrasion monoclonal anti-myoglobin antibiotic is acclimated for solid appearance apathy (on the microtiter wells). A dupe anti-myoglobin antibiotic is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The analysis sample is accustomed to acknowledge accompanying with the two antibodies, constant in the myoglobin molecules getting sandwiched amid the solid appearance and enzyme-linked antibodies. Afterwards a 45 minute evolution at allowance temperature, the wells are done with baptize to abolish absolved labeled antibodies. A TMB (Tetramethyl-benzidine) Reagent is added and incubated for 20 minutes, constant in the development of a dejected color. The blush development is chock-full with the accession of Stop Band-aid alteration the blush to yellow. The absorption of myoglobin is anon proportional to the blush acuteness of the analysis sample. Absorbance is abstinent spectrophotometrically at 450 nm.
Summary and Explanation of Test: Storage and Stability: 1. Store the kit at 2 - 8 C.
2. Keep microwells closed in a dry bag with desiccants.
3. The reagents are abiding until cessation of the kit.
4. Do not betrayal analysis reagents to heat, sun or able light
Storage and Stability: 1. Store the kit at 2 - 8 C.
2. Keep microwells closed in a dry bag with desiccants.
3. The reagents are abiding until cessation of the kit.
4. Do not betrayal analysis reagents to heat, sun or able light
Intended Use: The GenWay, Inc. Myoglobin ELISA Kit is advised for the quantitative assurance of Myoglobin in animal serum or plasma.
from:Medicine Kit